Sedative and cardiorespiratory effects of medetomidine, medetomidine-butorphanol, and medetomidine-ketamine in dogs   总被引：1，自引：0，他引：1  

Ko JC  Fox SM  Mandsager RE 《Journal of the American Veterinary Medical Association》2000,216(10):1578-1583

OBJECTIVE: To determine sedative and cardiorespiratory effects of i.m. administration of medetomidine alone and in combination with butorphanol or ketamine in dogs. DESIGN: Randomized, crossover study. ANIMALS: 6 healthy adult dogs. PROCEDURES: Dogs were given medetomidine alone (30 micrograms/kg [13.6 micrograms/lb] of body weight, i.m.), a combination of medetomidine (30 micrograms/kg, i.m.) and butorphanol (0.2 mg/kg [0.09 mg/lb], i.m.), or a combination of medetomidine (30 micrograms/kg, i.m.) and ketamine (3 mg/kg [1.36 mg/lb], i.m.). Treatments were administered in random order with a minimum of 1 week between treatments. Glycopyrrolate was given at the same time. Atipamezole (150 micrograms/kg [68 micrograms/lb], i.m.) was given 40 minutes after administration of medetomidine. RESULTS: All but 1 dog (given medetomidine alone) assumed lateral recumbency within 6 minutes after drug administration. Endotracheal intubation was significantly more difficult when dogs were given medetomidine alone than when given medetomidine and butorphanol. At all evaluation times, percentages of dogs with positive responses to tail clamping or to needle pricks in the cervical region, shoulder region, abdominal region, or hindquarters were not significantly different among drug treatments. The Paco2 was significantly higher and the arterial pH and Pao2 were significantly lower when dogs were given medetomidine and butorphanol or medetomidine and ketamine than when they were given medetomidine alone. Recovery quality following atipamezole administration was unsatisfactory in 1 dog when given medetomidine and ketamine. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggested that a combination of medetomidine with butorphanol or ketamine resulted in more reliable and uniform sedation in dogs than did medetomidine alone.  相似文献   

OsPPR9 encodes a DYW-type PPR protein that affects editing efficiency of multiple RNA editing sites and is essential for chloroplast development          下载免费PDF全文

CHEN Chang-zhao  WANG Ya-Liang  HE Meng-xing  LI Zhi-wen  SHEN Lan  LI Qing  RE De-yong  HU Jiang  ZHU Li  ZHANG Guang-heng  GAO Zhen-yu  ZENG Da-li  GUO Long-biao  QIAN Qian  ZHANG Qiang 《农业科学学报》2023,22(4):972-980

Photosynthesis occurs mainly in chloroplasts, whose development is regulated by proteins encoded by nuclear genes.  Among them, pentapeptide repeat (PPR) proteins participate in organelle RNA editing.  Although there are more than 450 members of the PPR protein family in rice, only a few affect RNA editing in rice chloroplasts.  Gene editing technology has created new rice germplasm and mutants, which could be used for rice breeding and gene function study.  This study evaluated the functions of OsPPR9 in chloroplast RNA editing in rice.  The osppr9 mutants were obtained by CRISPR/Cas9, which showed yellowing leaves and a lethal phenotype, with suppressed expression of genes associated with chloroplast development and accumulation of photosynthetic-related proteins.  In addition, loss of OsPPR9 protein function reduces the editing efficiency of rps8-C182, rpoC2-C4106, rps14-C80, and ndhB-C611 RNA editing sites, which affects chloroplast growth and development in rice.  Our data showed that OsPPR9 is highly expressed in rice leaves and encodes a DYW-PPR protein localized in chloroplasts.  Besides, the OsPPR9 protein was shown to interact with OsMORF2 and OsMORF9.  Together, our findings provide insights into the role of the PPR protein in regulating chloroplast development in rice.   相似文献   

Pulmonary adiaspiromycosis in critically endangered northern hairy‐nosed wombats (Lasiorhinus krefftii)          下载免费PDF全文

AB Schaffer‐White  D Harper  M Mayhew  A McKinnon  L Knott  RE Allavena 《Australian veterinary journal》2017,95(11):431-436

  相似文献   

克拉玛依果树腐烂病病原菌鉴定及其拮抗菌的筛选     

牛春林  叶晓芸  艾尼  王振华  热依汗  姜国勇  高雁  宋素琴  楚敏 《新疆农业科学》2018,55(2):304-311

【目的】提高克拉玛依果树腐烂病生物防治的效率,为相关果树高效生物防治提供科学依据和物质基础。【方法】从克拉玛依林杨的苹果树和李子树腐烂病发病植株及周边采集病灶和土壤样品,分析病原菌及其拮抗菌的筛选及微生物分子鉴定及拮抗效果。【结果】苹果树和李子树腐烂病病原菌均黑腐皮壳菌属（Valsa）,其中李子树病原菌涉及该属的两个种：Valsa mali 和Valsa leucostoma ,而苹果树病原菌仅为Valsa mali。同时获得各类真菌拮抗菌15株,涉及4个属9个种,筛选到具有高效拮抗细菌2株,其最大抑菌圈菌落比分别达到2-11和2-79。【结论】菌株芽孢杆菌B4和B6对果树腐烂病具有较好的抑菌效果,研究报道了李子树腐烂病病原菌,为相关果树的高效生物防治提供了科学依据和物质基础。  相似文献   

Comparison of a competitive inhibition ELISA and the card agglutination test for detection of antibodies to Anaplasma marginale and Anaplasma centrale in cattle     

JB MOLLOY  PM BOWLES  DP KNOWLES  TF McELWAIN  RE BOCK  TG KINGSTON  GW BLIGHT  RJ DALGLIESH 《Australian veterinary journal》1999,77(4):245-249

OBJECTIVE: To compare a recently developed recombinant MSP-5 competitive inhibition ELISA with a card agglutination test for detection of antibodies to Anaplasma marginale and Anaplasma centrale in Australian cattle. MATERIALS AND METHODS: The ELISA was compared with the card agglutination test using 208 sera from cattle in Anaplasma-free herds, 86 sera from cattle experimentally infected with A marginale or A centrale and 757 sera from cattle in areas endemic for A marginale. RESULTS: The specificity of the ELISA, based on testing 208 sera from cattle in Anaplasma-free areas, was 99.5%, and the sensitivities for detection of antibodies to A marginale and A centrale in sera from the experimentally infected cattle were 98.0% and 100%, respectively. For the same sets of sera, the specificity of the card agglutination test was 98.6% and the sensitivities for detection of antibodies to A marginale and A centrale were 98.0% and 100%, respectively. For the 757 sera collected from cattle in areas endemic for A marginale, the agreement between the ELISA and the card agglutination test depended on the positive threshold selected for the ELISA. The maximum achievable agreement was 91.5% (kappa = 0.73; 95% confidence interval 0.66, 0.79). CONCLUSION: We conclude that the competitive inhibition ELISA is a useful alternative to the card agglutination test for detection of A marginale or A centrale infection in cattle. The assay should be particularly useful for epidemiological applications such as prevalence studies and control programs.  相似文献   

Simulated increase of hurricane intensities in a CO2-warmed climate     

TR Knutson  RE Tuleya  Y Kurihara 《Science (New York, N.Y.)》1998,279(5353):1018-1020

Hurricanes can inflict catastrophic property damage and loss of human life. Thus, it is important to determine how the character of these powerful storms could change in response to greenhouse gas-induced global warming. The impact of climate warming on hurricane intensities was investigated with a regional, high-resolution, hurricane prediction model. In a case study, 51 western Pacific storm cases under present-day climate conditions were compared with 51 storm cases under high-CO2 conditions. More idealized experiments were also performed. The large-scale initial conditions were derived from a global climate model. For a sea surface temperature warming of about 2.2 degrees C, the simulations yielded hurricanes that were more intense by 3 to 7 meters per second (5 to 12 percent) for wind speed and 7 to 20 millibars for central surface pressure.  相似文献   

巴旦杏砧木组织培养及植株再生   总被引：7，自引：2，他引：5  

曾斌  克热木·伊力  郑华云  侯江涛 《经济林研究》2005,23(2):21-23

以新疆南疆咯什、和田地区的巴旦杏砧木品种桃巴旦、石头巴旦树上当年结的种子为试材,先用赤霉素打破休眠,消毒后接种子诱导生长及分化的培养基上。生长约一个月后,将由试管中种子培养长成的小植株剪切成长约1cm的单芽茎段,接入增殖培养基,20d后将增殖伸长的嫩茎用于生根,再将生根后的巴旦杏苗移栽到珍珠岩基质中培养,进行有效的移栽驯化管理,获得了较理想的移栽成活率。成功地进行了离体组织培养及植株再生。  相似文献   

养殖和野生亚东鲑机体成分比较分析          下载免费PDF全文

李琳  张斐然  刘长琳  刘宝良  李杰  多吉欧珠  热旦  德吉措姆  措姆  卫育良  梁萌青  徐后国 《渔业科学进展》2023,44(2):77-86

亚东鲑(Salmo trutta fario)是鲑属鱼类在青藏高原仅有的鱼类种群,是亚东地区全国农产品地理标志产品。本研究对西藏亚东地区野生和养殖亚东鲑的常规营养成分及各组织氨基酸和脂肪酸组成进行分析,旨在比较野生和养殖亚东鲑营养成分的异同,为养殖亚东鲑的品质评价和饲料配方的完善提供参考信息。实验采集亚东河中野生亚东鲑和亚东渔业产业园中使用配合饲料养殖的亚东鲑各10尾用于相关成分分析,每尾为一个独立样本。结果显示,野生组肥满度显著低于养殖组,而全鱼水分和灰分显著高于养殖组。全鱼粗蛋白、粗脂肪以及肝脏常规成分在野生组和养殖组间无显著差异。野生组肌肉粗脂肪显著低于养殖组,而水分含量显著高于养殖组。野生组全鱼必需氨基酸总量显著高于养殖组,且野生组肌肉中苏氨酸、缬氨酸、苯丙氨酸、赖氨酸和甘氨酸含量显著高于养殖组。野生组全鱼、肌肉和肝脏中饱和脂肪酸、n-3多不饱和脂肪酸(n-3 PUFA)总量、EPA、C20:4n-6以及肌肉中DHA含量高于养殖组,而全鱼和组织中单不饱和脂肪酸和n-6 PUFA总量低于养殖组。综上所述,目前养殖亚东鲑和野生亚东鲑在机体成分上存在较大差异。由于养殖鱼类体成分很大程度上反映了饲料组成,因此,亚东鲑养殖中饲料营养组成可能有待进一步优化。  相似文献   

提高造林“两率”问题的探讨     

王毅军  白永忠  王强  热希 《内蒙古林业调查设计》2012,(6):15-16

内蒙古乌拉特前旗属干旱、半干旱地区,地形、地貌复杂,土壤类型多样,造林立地条件较差。恶劣的自然条件,严重制约着造林成活率和保存率的提高。文章对当前制约该区造林成活率、保存率的因素进行调查分析,提出提高"两率"的具体措施,可为同类地区提供造林技术参考。  相似文献   

论沙化土地的治理     

白永忠  王毅军  张静  热希 《内蒙古林业调查设计》2012,(6):17-18

乌拉特前旗属干旱、半干旱地区,地貌类型复杂多样,造林立地条件较差。恶劣的自然条件,使大量土地日趋沙化,直接影响着这些地区的造林成活率和保存率的提高。文章对当前制约造林的沙化土地因素进行调查分析,提出治理的具体措施,可为同类地区提供造林技术参考。  相似文献